![]() More than 99% of human rabies cases are caused by rabid dogs, and control of rabies in dogs can reduce human cases. Current estimates suggest over 59,000 people die of rabies each year across the world. The vast majority of human rabies deaths are caused by a virus in the species Rabies lyssavirus, commonly referred to as rabies virus. Rabies is a progressive, fatal encephalitis caused by members of 17 different viral species within the Lyssavirus genus of rhabdoviruses. ![]() Taken together, our evaluation highlights the MinION’s potential for low-cost, high volume sequencing of pathogens in locations with limited resources. Phylogenetic analysis provided valuable insight into rabies virus diversity and distribution in these countries and identified a new rabies virus lineage in Kenya, the first published canine rabies virus sequence from Guatemala, evidence of rabies spread across an international border in Vietnam, and importation of a rabid dog into a state working to become rabies-free in India. A total of 259 sequences were generated from diverse rabies virus isolates in public health laboratories lacking rabies virus sequencing capacity in Guatemala, India, Kenya, and Vietnam. We developed a low-cost, high throughput rabies virus sequencing method using the Oxford Nanopore MinION portable sequencer. However, many rabies testing laboratories lack the resources or expertise for sequencing, especially in remote or rural areas where human rabies deaths are highest. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus variants that may have different host reservoir species or geographic distributions. (16) Almost every day that week, Pakistani-army minions carried out horrific acts on Indians.As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. (15) Probably more to the point, he was in charge, and didn't want comments from any minions who might happen to notice. ![]() (13) Is that what you'd do if you wanted to command your minions in the good fight? (14) Minor minions also decided to get in on the act of being totally dumbfounded as to what to do. (12) The days when they and their minions ran huge chunks of Britain's nationalised manufacturing capacity are long gone. ![]() (11) One of their minions appeared and told me that the judges had ruled against my appearance and he had instructions to drive me back to my hotel. (10) I am also making friends with everyone else, all the minions, so they can be on my side when it goes wrong. (9) If the United Kingdom is as bad as you teach your minions, then you have no reason to live here. (8) In his defence, it could be argued that a party leader ought to run up bigger expenses than his minions. (7) Probably he did, but Henry VIII won instant popularity for beheading his father's minions. (6) It was especially interesting to see how these minions went into overdrive after the recent Loyalist riots. (3) And when, asked a minion, might these changes kick in? (4) Maybe send a minion over to knock on the door once in a while? (5) As a middle-ranking minion in a large organisation, I am compelled to go to a lot of meetings. (2) I did point out to the minion who rang me that come next spring we were likely to be in a position to have a balance somewhere around the quarter million mark. (1) I was wary of her when she first started as the minion of our group at work but we became close through our appreciation of food and sarcastic wit, which no one else quite understood.
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